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Amaxa p3 primary cell 4d nucleofector x kit
P3 Primary Cell 4d Nucleofector X Kit, supplied by Amaxa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p3+primary+cell+nucleofector+kit/electroporation/bio_rxiv__64898__2026__03__27__714789-395-12-11
Average 86 stars, based on 1 article reviews
p3 primary cell 4d nucleofector x kit - by Bioz Stars, 2026-09
86/100 stars

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Article Title: Establishment of a human induced pluripotent stem cell line from a patient with Alagille syndrome carrying heterozygous mutation in JAG1 gene.
Article Snippet: Reprogramming was done using Amaxa P3 Primary Cell Nucleofector Kit following the manufacturer's instruction.

Article Title: Establishment of a non-integrated iPSC line (SDQLCHi043-A) from a male infant with propionic acidemia carrying compound heterozygote mutations in PCCB gene.
Article Snippet: 7 days later, 2 × 106 PBMCs were electroplated by Amaxa P3 Primary Cell Nucleofector Kit with episomal vectors (NovoBiotechnology) with 4D Nucleofector System (Lonza, program EO-100).

Article Title: Establishment of a non-integrated iPSC (SDQLCHi066-A) line derived from Segawa syndrome patients harboring heterozygous mutations in the TH gene (p.G247S and p.D491H).
Article Snippet: Subsequently, the cells were resuspended in 100 μl of nucleofection solution containing Amaxa P3 Primary Cell Nucleofector Kit and additional plasmids (1 μg pEV-SFFV-KLF4-Wpre, 2 μg pEV-SFFV-OCT4-E2ASOX2-Wpre, 0.5 μg pEV-SFFV-BCL-XL-Wpre, and 1 μg pEV-SFFV-MycWpre from NovoBiotechnology).

Article Title: Establishment of a transgene-free iPS cell line (SDCHi002-A) from a young patient bearing a NPRL3 mutation and suffering from Epilepsy.
Article Snippet: Then, PBMCs were electro transferred with Amaxa P3 Primary Cell Nucleofector Kit with episomal vectors (NovoBiotechnology) according to the standard protocol of 4D Nucleofector System (Lonza, program EO-100).

Article Title: Establishment of a non-integrated iPS cell line (SDQLCHi072-A) from a patient suffered from AUTS2 syndrome.
Article Snippet: In this study, PBMCs used in reprogramming were from a boy suffered from AUTS2 syndrome confirmed by clinical and genetic diagnosis. iPSCs were established by non-integrated method, which carried AUTS2 heterozygous mutation.. The established iPSCs presented similar appearance and expressed pluripotent markers in mRNA and protein level.. Additionally, the iPSCs possessed favorable differentiative capacity in vivo and normal karyotype.

Article Title: Establishment of a transgene-free iPS cell line (SDCHi007-A) from a young patient bearing a ATP1A2 mutation and suffering from Epilepsy.
Article Snippet: Epilepsy is a chronic neurological disease.. Here we describe the generation of induced pluripotent stem cells (iPSCs) from a patient diagnosed as epilepsy caused by ATP1A2 gene mutation.. Induced pluripotent stem cells (iPSCs) were developed using non-integrating episomal vectors containing OCT4, SOX2, KLF4, BCL-XL and CMYC.

Article Title: Establishment of a human induced pluripotent stem cell line(SDQLCHi059-A)from a patient with congenital disorder of glycosylation carrying heterozygous mutation in MPI gene.
Article Snippet: PBMCs were collected and electroporated with targeting vectors including pEV-SFFV-OCT4-E2A-SOX2- Wpre, pEVSFFV-BLC-XL-Wpre, pEV-SFFV-KLF4-Wpre and pEV-SFFV-Myc-Wpre (Novobiotec, Beijing, China) using Amaxa P3 Primary Cell Nucleofector Kit following the manufacturer’s instruction of 4D Nucleofector System (Lonza, program EO-100).

Plasmid Preparation:

Article Title: An integration-free iPSC line SDQLCHi065-A from a patient with down syndrome, possessing a 47, XY,+21, inv(9)(p12q21),16qh + karyotype.
Article Snippet: .. The cells were suspended in a 100 μl nuclear transfer solution, which included Amaxa P3 Primary Cell Nucleofector Kit and an additive vector (1μg pEV-SFFV-KLF4-Wpre, 1μg PEV-SFFV-KLF4-WPRE, 2μg EV-SFFV-OCT4-E2A-SOX2-Wpre, 0.5μg pEV-SFFV-BCL-XL-Wpre, and 1μg pEV-SFFV-Myc-Wpre from NovoBiotechnology, Beijing, China). .. The mixture was transferred to a rotating cup and subjected to the standard protocol of the 4D Nucleofector System (Lonza, program EO-100).



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